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anti-ER antibody product blog

Posted on 2017-05-23 07:15:03 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-ER antibody;
The ER n/a (Catalog #MBS475748) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ER Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ER can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ER n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-ER Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot analysis using ER mouse mAb truncated ER recombinant protein (1) MCF-7 cell lysates (2).)
Western Blot (WB) ER.

Western Blot (WB) (Western blot analysis using ER mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ER cDNA (2).)
Western Blot (WB) ER.

Entrez Summary: ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites. Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7. Ser167 may be phosphorylated by p90RSK and Akt. Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients.

Immunogen: Purified recombinant fragment of ER expressed in E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ER are readily searchable from our website. Different antibodies against the same target such as ER may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.
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