anti-ERK2 antibody product blog
Tags: Antibody; Monoclonal Antibody; ERK2; anti-ERK2 antibody;
The ERK2 mapk1 (Catalog #MBS850453) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ERK2 Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s ERK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000. Researchers should empirically determine the suitability of the ERK2 mapk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ERK2 mapk1 product has the following accession number(s) (GI #66932916) (NCBI Accession #NP_002736.3) (Uniprot Accession #P28482). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ERK2 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1. Western blot analysis using ERK2 mouse mAb against Hela (1), NIH/3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate.)
Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and breast carcinoma (right) showing cytoplasmic localization using ERK2 mouse mAb with DAB staining.)
Immunofluorescence (IF) (Figure 3. Confocal immunofluorescence analysis of Eca-109 cells using ERK2 mouse mAb (green).)
ERK2 (also designated extracellular-signal-related kinase 2 or mitogen-activated protein kinase 1), with 360-amino acid protein (about 40kDa), belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.
Immunogen: Purified recombinant fragment of human ERK2 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ERK2 are readily searchable from our website. Different antibodies against the same target such as ERK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.