|The ERp57 pdia3 (Catalog #MBS808438) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ERp57 Antibody, Clone 4F9: ATTO 700 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ERp57 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).
ICC/IF: 1:100. Researchers should empirically determine the suitability of the ERp57 pdia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ERp57 pdia3 product has the following accession number(s) (GI #21361657) (NCBI Accession #NP_005304.3) (Uniprot Accession #P30101). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
Immunogen: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25- 505 (his-tagged)
Immunogen Species: Human
Cellular Localization: Endoplasmic Reticulum; Endoplasmic Reticulum Lumen; Melanosome
Conjugate: ATTO 700
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ERp57 are readily searchable from our website. Different antibodies against the same target such as ERp57 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Breast Neoplasms, Carcinoma, Hepatocellular, Cardiovascular Diseases, Disease Models, Animal, Immune System Diseases, Inflammation, Liver Diseases, Liver Neoplasms, Necrosis, Nervous System Diseases are some of the diseases may be linked to ERp57 Antibody, Clone 4F9: ATTO 700.