|The Espl1 espl1 (Catalog #MBS6001152) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ESPL1 (Separin, Caspase-like Protein ESPL1, Extra Spindle Poles-like 1 Protein, ESP1, KIAA0165) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ESPL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the Espl1 espl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Espl1 espl1 product has the following accession number(s) (GI #148877887) (NCBI Accession #AAI45845.1) (Uniprot Accession #P60330). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
REELQAYKAV RADTGQERFN IICDLLELSP EETPAGAWAR ATHLVELAQV LCYHDFTQQT NCSALDAIRE ALQLLDSVRP EAQARDQLLD DKAQALLWLY ICTLEAKIQE GIERDR.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
ESPL1 is a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. ESPL1 is regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. The association with PTTG1 is not only inhibitory, since PTTG1 is also required for activating it, the enzyme being inactive in cells in which PTTG1 is absent. PTTG1 degradation at anaphase, liberates it and triggers RAD21 cleavage. Second, phosphorylation at Ser-1126 inactivates it. The complete phosphorylation during mitosis, is removed when cells undergo anaphase. Activation of the enzyme at the metaphase-anaphase transition probably requires the removal of both securin and inhibitory phosphate.
Immunogen: Partial recombinant corresponding to aa586-701 from ESPL1 (NP_036423) with GST tag. MW of the GST tag alone is 26kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Espl1 are readily searchable from our website. Different antibodies against the same target such as Espl1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Bone Marrow, Eye, Kidney, Lymph Node, Mammary Gland, Thymus tissues are correlated with this protein.