anti-ESR1 antibody product blog
Tags: Antibody; Monoclonal Antibody; ESR1; anti-ESR1 antibody; Estrogen Receptor, alpha;
The ESR1 esr1 (Catalog #MBS632361) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Estrogen Receptor, alpha, phosphorylated (Ser118) (ER) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Estrogen Receptor, alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Dilution: Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunohistochemistry (Paraffin): 1:50. Researchers should empirically determine the suitability of the ESR1 esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ESR1 esr1 product has the following accession number(s) (GI #62821794) (NCBI Accession #NP_000116.2) (Uniprot Accession #P03372). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Estrogen Receptor, alpha, phosphorylated (Ser118) (ER) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from untransfected MCF-7 cells, and COS-1 cells transfected with wild-type or mutant ER alpha, stimulated with EGF and E2, using MBS632361 or control estrogen receptor alpha antibody (lower).)
Immunohistochemistry (IHC) (Immunohistochemical staining of phosphorylated estrogen receptor alpha in paraffin-embedded human breast carcinoma showing nuclear localization, using MBS632361.)
Estrogen receptor alpha (ER alpha), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER alpha regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ER alpha activity (3,4). ER alpha is phosphorylated on multiple sites (5). Serines 104, 106, 118 and 167 are located in the N-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER alpha activity. Ser118 may be the substrate of the transcription regulatory kinase cdk7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).
Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser118 of human ER alpha. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ESR1 are readily searchable from our website. Different antibodies against the same target such as ESR1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.