anti-F12 antibody product blog
Tags: Antibody; Monoclonal Antibody; F12; Factor XII; anti-F12 antibody;
The F12 f12 (Catalog #MBS2541908) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The F12 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s F12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA).WB: 1:500-1:5000
IHC: 1:50-1:500
IF: 1:50-1:500. Researchers should empirically determine the suitability of the F12 f12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The F12 f12 product has the following accession number(s) (GI #145275213) (NCBI Accession #NP_000496.2) (Uniprot Accession #P00748). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the F12 Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of K-562 cells using Factor XII Monoclonal Antibody at dilution of 1:1000)
Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. When plasma comes into contact with negatively charged surfaces such as glass, kaolin, or dextran sulfate, F XII is bound to the surface and undergoes limited proteolysis by plasma kallikrein, resulting in two active enzyme forms, ?-F XIIa and beta-F XIIa. ?-F XIIa consisting of a 28kD light chain containing the protease domain, and a 52kD heavy chain containing the anionic surface-binding domain. beta-F XIIa consists of two disulfide-linked chains, a 2 kD light chain and a 28 kD heavy chain containing the catalytic domain. Defects in Factor XII do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.
Immunogen: Fusion protein of Factor XII. Buffer: PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation: Unconjugated. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing F12 are readily searchable from our website. Different antibodies against the same target such as F12 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Cell Transformation, Neoplastic, Fetal Death, Fetal Diseases, Gastrointestinal Diseases, Liver Diseases, Liver Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to F12 Monoclonal Antibody.