|The FADD fadd (Catalog #MBS475356) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-FADD Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s FADD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).
WB: 1:500. Researchers should empirically determine the suitability of the FADD fadd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FADD fadd product has the following accession number(s) (GI #4505229) (NCBI Accession #NP_003815.1) (Uniprot Accession #Q13158). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-FADD Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of FADD in SW480, Jurkat and Hela cell lysates using FADD mouse mAb (dilution 1:500).Predicted band size:28kDa.Observed band size:28kDa.)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using FADD mouse mAb.)
Entrez Summary: The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals.Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors.Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade.Knockout studies in mice also suggest the importance of this protein in early T cell development.[provided by RefSeq, Jul 2008].
Immunogen: Recombinant human FADD protein.
Sensitivity: This antibody detects endogenous levels of FADD and does not cross-react with related proteins.
Sequence Similarities: Contains 1 death domain.Contains 1 DED (death effector) domain. Subunit Structure: Can self-associate. Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and PIDD1. Interacts (via death domain) with FAS (via death domain). Interacts with CASP8. Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (By similarity). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FADD are readily searchable from our website. Different antibodies against the same target such as FADD may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.