anti-FEN1 antibody product blog
Tags: Antibody; Monoclonal Antibody; FEN1 ; anti-FEN1 antibody; Fen-1;
The FEN1 fen1 (Catalog #MBS9215548) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FEN-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s FEN-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).WB ~~ 1:1000
ICC ~~ 1:400. Researchers should empirically determine the suitability of the FEN1 fen1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FEN1 fen1 product has the following accession number(s) (GI #4758356) (NCBI Accession #NP_004102.1) (Uniprot Accession #P39748). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FEN-1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.)
Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).)
Structure-specific nuclease with 5\'-flap endonuclease and 5\'-3\' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5\'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5\'-end of a downstream Okazaki fragment. It enters the flap from the 5\'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5\'-3\' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Antigen Source: Human. Antigen Type: Recombinant Protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FEN1 are readily searchable from our website. Different antibodies against the same target such as FEN1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. FEN1 also interacts with the following gene(s): APEX1, BLM, DDX11, DNA2, MRE11A, PCNA, RAD52, RPA1, WRN.