anti-FHL2 antibody product blog
Tags: Antibody; Monoclonal Antibody; FHL2; anti-FHL2 antibody;
The FHL2 fhl2 (Catalog #MBS584089) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s FHL2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB).Dilutions to be used depend on detection system applied.
It is recommended that users test the reagent and determine their own optimal dilutions.
The typical starting working dilution is 1:10.
IF: MBS584089 is useful for immunofluorescence on paraformaldehyde fixed cells. Researchers should empirically determine the suitability of the FHL2 fhl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FHL2 fhl2 product has the following accession number(s) (GI #87159836) (NCBI Accession #NP_001034581.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FHL2 are readily searchable from our website. Different antibodies against the same target such as FHL2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.