anti-Fibroblast Growth Factor-21 antibody product blog
Tags: Antibody; Monoclonal Antibody; Fibroblast Growth Factor-21; anti-Fibroblast Growth Factor-21 antibody;
The Fibroblast Growth Factor-21 n/a (Catalog #MBS606888) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fibroblast Growth Factor-21 (FGF-21) (UNQ3115/PRO10196) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Fibroblast Growth Factor-21 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry, Immunocytochemistry and ELISA.,, Recommended Dilution:, Flow Cytometry: 25ug/ml; 10ul labels 1-5x10e5 HepG2 cells in a total reaction volume <200ul. For intercellular staining, cells must be fixed and permeabilized using PBS, 4% paraformaldehyde, 0.1% saponin., Immunocytochemistry: 10ug/ml; HepG2 human cells were fixed PBS, 4% paraformaldehyde for 20 min at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-10, 1% BSA for 45 min at RT. Cells were incubated with F4212-93E overnight at 4ºC follwed by IgG (FITC) anti-mouse* at RT in the dark for 1 hour. Cells were washed with PBS, 0.1% BSA between each step., Direct ELISA: 0.5-1ug/ml; The detection limit is ~1ng/well., Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Fibroblast Growth Factor-21 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Fibroblast Growth Factor-21 n/a product has the following accession number(s) (GI #114731554). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Fibroblast Growth Factor-21 (FGF-21) (UNQ3115/PRO10196) with the following immunoassay(s):
Testing Data (HepG2 cells were stained with MBS606888 (filled histogram) or isotype control (open histogram) followed by |APC-conjugated anti-mouse antibody.)
Fibroblast Growth Factor-21 (FGF-21) belongs to the FGF family of heparin-binding polypeptides and is most closely related to FGF-23. Both proteins are synthesized as a precursor with hydrophobic signal peptides. FGF-21 is most highly expressed in the liver. Mature human FGF-21 shares 82%aa sequence homology with mature mouse FGF-21.
Immunogen: Recombinant corresponding to aa29-209 of human FGF-21 expressed in E. coli (Q9NSA1). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Fibroblast Growth Factor-21 are readily searchable from our website. Different antibodies against the same target such as Fibroblast Growth Factor-21 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Fibroblast Growth Factor-21 also interacts with the following gene(s): FLNA. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.