anti-FIBROBLASTS/EPITHELIAL CELLS antibody product blog
Tags: Antibody; Monoclonal Antibody; FIBROBLASTS/EPITHELIAL CELLS; anti-FIBROBLASTS/EPITHELIAL CELLS antibody;
The FIBROBLASTS/EPITHELIAL CELLS n/a (Catalog #MBS214994) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s FIBROBLASTS/EPITHELIAL CELLS can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the FIBROBLASTS/EPITHELIAL CELLS n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS:RPE with the following immunoassay(s):
Testing Data (Staining of KG1 cells with Mouse anti Human Fibroblasts/ Epithelial cells (MBS214991))
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001). Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.
Reconstitution: Reconstitute in 0.25 ml disilled water
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Human foreskin fibroblasts
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2 myeloma cell line
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FIBROBLASTS/EPITHELIAL CELLS are readily searchable from our website. Different antibodies against the same target such as FIBROBLASTS/EPITHELIAL CELLS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.