|The FLAG flag (Catalog #MBS533391) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s FLAG can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB).
WB: 1:1000-3000. Researchers should empirically determine the suitability of the FLAG flag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The FLAG flag product has the following accession number(s) (GI #359759500) (NCBI Accession #AEV61579.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FLAG antibody with the following immunoassay(s):
Western Blot (Western Blot analysis using FLAG antibody
1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with DYKDDDDK-tagged protein vector; unstranfected (1) and transfected (2).)
Mouse monoclonal FLAG antibody.
Biological Significance: FLAG tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Immunogen: FLAG antibody was raised in mouse using DYKDDDDK (FLAG) synthetic peptide conjugated to KLH as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing FLAG are readily searchable from our website. Different antibodies against the same target such as FLAG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.