anti-Flag tag antibody product blog
Tags: Antibody; Monoclonal Antibody; FLAG Tag; anti-FLAG Tag antibody;
The DYKDDDDK tag n/a (Catalog #MBS206620) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DYKDDDDK tag reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s DYKDDDDK tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the DYKDDDDK tag n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the DYKDDDDK tag with the following immunoassay(s):
Western Blot (WB) (The recombinant proteins (200ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYKDDDDK tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant Human 6XHis-SNCA-3xFlag
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYKDDDDK tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: ODC1-DYKDDDDK tag Transfected 293T cell lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYKDDDDK tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HDAC2-DYKDDDDK tag Transfected 293T cell lysate)
DYKDDDDK-tag, or DYKDDDDK octapeptide, or DYKDDDDK epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It is an artificial antigen to which specific, high affinity monoclonal antibodies have been developed and hence can be used for protein purification by affinity chromatography and also can be used for locating proteins with in living cells. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A DYKDDDDK-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a DYKDDDDK-tag to a protein allows the protein to be studied with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.