|The Fn14 tnfrsf12a (Catalog #MBS832619) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Fn14 can be used in a range of immunoassay formats including, but not limited to, Functional Assay.
The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/ml. This corresponds to a specific activity of greater than or equal to 4 x 10e7 Units/mg. Researchers should empirically determine the suitability of the Fn14 tnfrsf12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Fn14 tnfrsf12a product has the following accession number(s) (GI #38488982) (NCBI Accession #AAR21225.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Fn14 antibody with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a kappa Isotype Control (open histogram) or 0.5 ug CD266 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Total viable cells were analyzed.)
Mouse monoclonal Fn14 antibody.
Biological Significance: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14. Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Fn14 are readily searchable from our website. Different antibodies against the same target such as Fn14 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.