anti-FYN antibody product blog
Tags: Antibody; anti-Fyn antibody; Fyn; Monoclonal Antibody;
The Fyn fyn (Catalog #MBS475169) is an Antibody produced from MouseSource: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Fyn Mouse mAb reacts with Human, Monkey, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Fyn can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
WB: 1:500
ICC: 1:50. Researchers should empirically determine the suitability of the Fyn fyn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Fyn fyn product has the following accession number(s) (GI #4503823) (NCBI Accession #NP_002028.1) (Uniprot Accession #P06241). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Fyn Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of Fyn in Hela, 3T3, C6, COS7, CEM, Ramos and Jurkat cell lysates using Fyn mouse mAb (1:500 diluted).Predicted band size:59KDa.Observed band size:59KDa.)
Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with -20 degree C Methanol and using Fyn mouse mAb (dilution 1:50).)
Entrez Summary: This gene is a member of the protein-tyrosine kinase oncogene family.It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth.The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein.Alternatively spliced transcript variants encoding distinct isoforms exist.[provided by RefSeq, Jul 2008].
Immunogen: Purified recombinant human Fyn protein fragments expressed in E Coli.
Sensitivity: This antibody detects endogenous levels of Fyn and does not cross-react with related proteins.
Sequence Similarities: Belongs to the protein kinase superfamily.Tyr protein kinase family.SRC subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain. Subunit Structure: Interacts (via its SH3 domain) with PIK3R1 and PRMT8.Interacts with FYB, PAG1, and SH2D1A.Interacts with CD79A (tyrosine-phosphorylated form); the interaction increases FYN activity.Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated) by similarity.Interacts with TOM1L1 (phosphorylated form).Interacts with KDR (tyrosine phosphorylated).Interacts (via SH3 domain) with KLHL2 (via N-terminus) by similarity.Interacts with SH2D1A and SLAMF1.Interacts (via its SH3 domain) with HEV ORF3 protein.Interacts with ITCH; the interaction phosphorylates ITCH and negatively regulates its activity.Interacts with FASLG.Interacts with RUNX3.Interacts with KIT.Interacts with EPHA8; possible downstream effector of EPHA8 in regulation of cell adhesion.Interacts with PTK2/FAK1; this interaction leads to PTK2/FAK1 phosphorylation and activation.Interacts with CAV1; this interaction couples integrins to the Ras-ERK pathway.
Subcellular Location: Cytoplasm. Nucleus. Cell membrane. Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.