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anti-GABA-A antibody product blog

Posted on 2016-04-24 20:41:09 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-GABA-A antibody; GABA-A; GABA-A Receptor Alpha5;
The GABA-A gabra5 (Catalog #MBS807152) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human GABA-A Receptor Alpha5 Monoclonal IgG1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GABA-A Receptor Alpha5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).
Western Blot: 1:1000. Researchers should empirically determine the suitability of the GABA-A gabra5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GABA-A gabra5 product has the following accession number(s) (GI #4503861) (NCBI Accession #NP_000801.1) (Uniprot Accession #P31644). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Human GABA-A Receptor Alpha5 Monoclonal IgG1 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24. Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.)
Western Blot (WB) GABA-A.

The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer\'s Disease (3).

Immunogen: Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA-A Receptor Alpha5.
Immunogen Species: Human. COA: A 1:100 dilution was sufficient for detection of GABA-A R, Alpha5 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
Conjugate: Streptavidin
Cellular Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GABA-An are readily searchable from our website. Different antibodies against the same target such as GABA-A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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