|The Gabbr1 gabbr1 (Catalog #MBS800614) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GABA-B Receptor 1 Antibody: RPE reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GABA(B) Receptor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1:1000 (WB). Researchers should empirically determine the suitability of the Gabbr1 gabbr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Gabbr1 gabbr1 product has the following accession number(s) (GI #77404238) (NCBI Accession #NP_112290.2) (Uniprot Accession #Q9Z0U4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GABA-B Receptor 1 Antibody: RPE with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone S93A-49. Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody at 1:1000.)
Background Info: Detects ~115kDa. No cross-reactivity against GABA(Beta)R2.
Scientific Background: GABA (gamma-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
Conjugate: RPE. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Gabbr1 are readily searchable from our website. Different antibodies against the same target such as Gabbr1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.