anti-Gabbr2 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-Gabbr2 antibody; Gabbr2; GABA(B) Receptor 2;
The Gabbr2 gabbr2 (Catalog #MBS801833) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GABA-B Receptor 2 Antibody: ATTO 594 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GABA(B) Receptor 2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunocytochemistry (ICC).1:1000 (WB). Researchers should empirically determine the suitability of the Gabbr2 gabbr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Gabbr2 gabbr2 product has the following accession number(s) (GI #13929138) (NCBI Accession #NP_113990.1) (Uniprot Accession #O88871). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GABA-B Receptor 2 Antibody: ATTO 594 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain Membrane showing detection of ~105 kDa GABA B Receptor 2 protein using Mouse Anti-GABA B Receptor 2 Monoclonal Antibody, Clone S81-2. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-GABA B Receptor 2 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~105 kDa.)
Background Info: Detects ~105kDa. No cross-reactivity against GABA(B)R1
Scientific Background: GABA (gamma-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).