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anti-Gabra1 antibody product blog

Posted on 2015-09-25 01:17:24 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-Gabra1 antibody; Gabra1; GABA(A) Receptor Alpha1;
The Gabra1 gabra1 (Catalog #MBS800004) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GABA-A Receptor Alpha-1 Antibody reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GABA(A) Receptor Alpha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF). Researchers should empirically determine the suitability of the Gabra1 gabra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Gabra1 gabra1 product has the following accession number(s) (GI #6753936) (NCBI Accession #NP_034380.1) (Uniprot Accession #P14867). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GABA-A Receptor Alpha-1 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.)
Immunohistochemistry (IHC) Gabra1.

Western Blot (WB) (Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S95-35. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB) Gabra1.

Background Info: Detects ~55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or -Alpha3.

Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).

Certificate of Analysis: 1 ug/mL was sufficient for detection of alpha1 GABA receptor in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Gabra1 are readily searchable from our website. Different antibodies against the same target such as Gabra1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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