anti-GAPDH antibody product blog
Tags: Antibody; GAPDH; Monoclonal Antibody; anti-GAPDH antibody;
The GAPDH gapdh (Catalog #MBS224740) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s GAPDH can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Western Blot (WB).Western Blot: This item is suitable for use as a loading control
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GAPDH gapdh product has the following accession number(s) (GI #378404908) (NCBI Accession #NP_001243728.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RABBIT GAPDH:HRP with the following immunoassay(s):
Testing Data #1 (Staining of HeLa whole cell lysate with Mouse anti Rabbit GAPDH:DylightR750 (MBS219746D750) imaged with a LiCor OdysseyR )
Testing Data #2 (Staining of HeLa whole cell lysate with Mouse anti Rabbit GAPDH:DylightR800 (MBS219848) imaged with a LiCor OdysseyR )
Testing Data #3 (Staining of HeLa whole cell lysate with Mouse anti Rabbit GAPDH:DylightR680 (MBS219808) imaged with a LiCor OdysseyR )
Testing Data #4 (Staining of HeLa whole cell lysate with Mouse anti Rabbit GAPDH:HRP (MBS224740))
Mouse anti Rabbit GAPDH antibody, clone 6C5 recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36kD multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism. GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin (Hara et al. 2006).
Perservative Stabilisers: 0.01% Thiomersal
HRP Stabiliser
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Rabbit. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GAPDH are readily searchable from our website. Different antibodies against the same target such as GAPDH may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.