|The GFAP gfap (Catalog #MBS804635) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GFAP R416WT Antibody: PerCP reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GFAP R416WT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC).
1:1000 WB. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GFAP gfap product has the following accession number(s) (GI #196115290) (NCBI Accession #NP_001124491.1) (Uniprot Accession #P14136). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GFAP R416WT Antibody: PerCP with the following immunoassay(s):
Testing Data (Western blot of rat brain membrane lysate showing detection of ~50 kDa GFAP R416WT protein using Anti-GFAP R416WT, clone S206B-9. Lane 1: MW ladder. Lane 2: Anti-GFAP R416WT (1:250). Load: 66 µg RBM lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Mouse Anti-GFAP R416WT, clone S206B-9 incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~ 50 kDa. Other band(s): none.)
Background Info: Detects a 50kDa protein in WB. Does not cross-react with GFAP-R416W or other proteins (based on KO validation results).
Scientific Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.
Conjugate: PerCP. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GFAP are readily searchable from our website. Different antibodies against the same target such as GFAP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.