anti-GLUL antibody product blog
Tags: Antibody; Monoclonal Antibody; GLUL; anti-GLUL antibody;
The GLUL glul (Catalog #MBS206590) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GLUL antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s GLUL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF).The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity.
Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the GLUL glul for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GLUL glul product has the following accession number(s) (GI #74271837) (NCBI Accession #NP_001028216) (Uniprot Accession #P15104). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GLUL antibody with the following immunoassay(s):
Western Blot (WB) (The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GLUL antibody (1:1000).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain tissue lysate
Lane 2.: Mouse liver tissue lysate)
Immunofluorescence (IF) (ICC/IF analysis of GLUL in HeLa cells.
The cell was stained with MBS206590 (1:100).
The secondary antibody (green) was used Alexa Fluor 488.
DAPI was stained the cell nucleus (blue).)
Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency.
Immunogen: Recombinant human GLUL (1-373aa) purified from E. coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GLUL are readily searchable from our website. Different antibodies against the same target such as GLUL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.