anti-GNL3 antibody product blog
Tags: Antibody; Monoclonal Antibody; HSP22; GNL3; anti-GNL3 antibody;
The GNL3 slc29a1 (Catalog #MBS804207) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp22 Antibody: Biotin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp22 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Immunofluorescence (IF).1:2000 (WB). Researchers should empirically determine the suitability of the GNL3 slc29a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GNL3 slc29a1 product has the following accession number(s) (NCBI Accession #NP_055180.2) (Uniprot Accession #Q9UJY2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsp22 Antibody: Biotin with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Granular layer of the epidermis. Some dermal staining.)
Western Blot (WB) (Western Blot analysis of Rat Cell lysates showing detection of Hsp22 protein using Mouse Anti-Hsp22 Monoclonal Antibody, Clone 3C12-H11. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp22 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin. Exogenous hsp22 detected in dimeric form.
Scientific Background: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).