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anti-Grk5 antibody product blog

Posted on 2015-09-17 12:57:58 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; GRK5; anti-GRK5 antibody; GRK (pan);
The Grk5 grk5 (Catalog #MBS803520) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GRK (pan) Antibody: RPE reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s GRK (pan) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1:1000 WB. Researchers should empirically determine the suitability of the Grk5 grk5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Grk5 grk5 product has the following accession number(s) (GI #159032059) (NCBI Accession #NP_061357.3) (Uniprot Accession #Q8VEB1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GRK (pan) Antibody: RPE with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS cells transfected with untagged GRK6 showing detection of ~65 kDa GRK (pan) protein using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with untagged GRK6. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~65 kDa.)
Western Blot (WB) Grk5.

Background Info: Detects a 70 kDa protein. Cross-reacts with GRK6.

Scientific Background: G protein-coupled receptor kinases (GRK) are serine/threonine kinases that phosphorylate a variety of G-protein-coupled receptors (GPCRs), specifically activated forms, including adrenergic receptors, muscarinic acetylcholine receptors, dopamine receptors and opioid receptors. GRK receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation.

Conjugate: RPE. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Grk5 are readily searchable from our website. Different antibodies against the same target such as Grk5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Cardiovascular Diseases, Disease Models, Animal, Fibrosis, Heart Diseases, Hypertension, Hypertrophy, Inflammation, Liver Diseases, Necrosis, Nervous System Diseases are some of the diseases may be linked to GRK (pan) Antibody: RPE.
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