anti-GRP94 antibody product blog
Tags: Antibody; Monoclonal Antibody; Grp94; anti-GRP94 antibody;
The GRP94 hsp90b1 (Catalog #MBS809116) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GRP94 Antibody, Clone 6B12: ATTO 488 reacts with Human; Mouse; Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s GRP94 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:1000. Researchers should empirically determine the suitability of the GRP94 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GRP94 hsp90b1 product has the following accession number(s) (GI #4507677) (NCBI Accession #NP_003290.1) (Uniprot Accession #P14625). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GRP94 Antibody, Clone 6B12: ATTO 488 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of 94 kDa GRP94 protein using Mouse Anti-GRP94 Monoclonal Antibody, Clone 6B12. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GRP94 Monoclonal Antibody at 1:1000 for O/N at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 94 kDa.)
Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5).
Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).