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anti-GSDMD antibody product blog

Posted on 2015-10-21 23:46:57 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; GSDMD; anti-GSDMD antibody; Gasdermin D / GSDMD;
The GSDMD gsdmd (Catalog #MBS246242) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 3F12-1B2] (IgG1,k) to Human Gasdermin D / GSDMD reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Gasdermin D / GSDMD can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control Jurkat. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). Researchers should empirically determine the suitability of the GSDMD gsdmd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The GSDMD gsdmd product has the following accession number(s) (GI #83320070) (NCBI Accession #NP_079012.3) (Uniprot Accession #P57764). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 3F12-1B2] (IgG1,k) to Human Gasdermin D / GSDMD with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-GSDMD / DFNA5L antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) GSDMD.

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to GSDMDC1 on HeLa cell (antibody concentration 10 ug/ml).)
Immunofluorescence (IF) GSDMD.

Western Blot (WB) (GSDMDC1 monoclonal antibody, clone 3F12-1B2 Western blot of GSDMDC1 expression in Jurkat.)
Western Blot (WB) GSDMD.

ELISA (EIA) (Detection limit for recombinant GST tagged GSDMDC1 is approximately 0.3 ng/ml as a capture antibody.)

Target Species: Human
Immunogen Description: GSDMDC1 (AAH08904, 1 a.a. ~ 485 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen Type: Recombinant protein
Immunogen: Gasdermin D / GSDMD antibody was raised against gSDMDC1 (AAH08904, 1 a.a. ~ 485 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GSDMD are readily searchable from our website. Different antibodies against the same target such as GSDMD may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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