anti-GSK3B antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-GSK3B antibody; GSK3B; glycogen synthase kinase 3 beta;
The GSK3B gsk3b (Catalog #MBS6000510) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Glycogen Synthase Kinase 3 beta, Phosphorylated (S9) (GSK-3b, GSK-3 beta, GSK3B) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Glycogen Synthase Kinase 3 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry and Western Blot.
Dilution: Flow Cytometry: 2.5ug labels 10e6 cells
Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the GSK3B gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GSK3B gsk3b product has the following accession number(s) (GI #5730335) (NCBI Accession #AAD48517.1) (Uniprot Accession #P49841). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Glycogen Synthase Kinase 3 beta, Phosphorylated (S9) (GSK-3b, GSK-3 beta, GSK3B) with the following immunoassay(s):
Western Blot (WB) (Detection of Human PhosphoGSK3b (S9) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated () or treated (+) with 200 nM PMA for 20 minutes. PVDF Membrane was probed with 1ug/ml of Human PhosphoGSK3b (S9) Monoclonal Antibody followed by HRP conjugated Mouse IgG Secondary Antibody. A specific band was detected for PhosphoGSK3b (S9) at approximately 46kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Flow Cytometry (FC/FACS) (Detection of PhosphoGSK3b (S9) in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line untreated (open histogram) or treated with 120 ng/mL PMA for 15 minutes (filled histogram) was stained with Human PhosphoGSK3b (S9) Monoclonal Antibody or isotype control antibody (, followed by Fluorescein conjugated Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.)
Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3a, GSK-3b) share 85aa identity. GSK-3b, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.
Immunogen: Synthetic peptide corresponding to human GSK-3b S9. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing GSK3B are readily searchable from our website. Different antibodies against the same target such as GSK3B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.