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anti-H3 antibody product blog

Posted on 2015-10-06 04:37:40 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; Histone H3; anti-H3 antibody;
The Histone H3 h3 (Catalog #MBS375166) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Histone H3 (tri methyl K9, di methyl K9) [6F12-H4] reacts with Reacts with Human and cow. predicted to detect many other species due to sequence identity such as Mouse, Rat, Rabbit, Hamster, Pig, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster), Non Human Primates and may cross-react with other species as described in the data sheet. MyBioSource\'s Histone H3 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB) against endogenous protein, ELISA (EIA), ChIP.
Optimal antibody dilution should be determined by titration, however as a guideline try up to 1:1000 for western blot.Detects a band of approximately 17 kDa (predicted molecular weight: 15 kDa).Can be blocked with Histone H3 peptide - tri methyl K9. Researchers should empirically determine the suitability of the Histone H3 h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Histone H3 h3 product has the following accession number(s) (GI #3273350) (NCBI Accession #BAA31218.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Histone H3 (tri methyl K9, di methyl K9) [6F12-H4] with the following immunoassay(s):
Western Blot (WB)
Western Blot (WB) Histone H3.

Target Antigen: Histone H3 (tri methyl K9, di methyl K9)
Immunogen: Synthetic peptide of Human Histone H3, tri methylated at K9. Myeloma/fusion Partners: X63-Ag8.653. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Histone H3 are readily searchable from our website. Different antibodies against the same target such as Histone H3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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