anti-HDEL antibody product blog
Tags: Antibody; Monoclonal Antibody; HDEL; anti-HDEL antibody;
The HDEL n/a (Catalog #MBS804858) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HDEL Antibody: ATTO 594 reacts with Drosophilia, Yeast, Saccharomyce, Barnyard grass, Beet, Cotton, Mung bean, Sorghum, Wheat and may cross-react with other species as described in the data sheet. MyBioSource\'s HDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).1:100-1:1000 (WB), 1:50-1:500 (IF). Researchers should empirically determine the suitability of the HDEL n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HDEL Antibody: ATTO 594 with the following immunoassay(s):
Testing Data (IHC analysis of HDEL tested using a human keratinocyte line, HaCat.
Courtesy of Dr. Turksen, Ottawa Research Hospital Institute, Canada.)
Background Info: Detects ~78kDa.
Scientific Background: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
Conjugate: ATTO 594. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HDEL are readily searchable from our website. Different antibodies against the same target such as HDEL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.