anti-Helicard; Melanoma differentiation-associated gene 5; Interferon-induced helicase C domain-containing protein 1; RH116; CADM-140 autoantigen; RIG-I-like receptor 2; RLR-2 antibody product blog
Tags: Antibody; Monoclonal Antibody; MDA5; anti-Helicard; Melanoma differentiation-associated gene 5; Interferon-induced helicase C domain-containing protein 1; RH116; CADM-140 autoantigen; RIG-I-like receptor 2; RLR-2 antibody; Helicard; RLR-2;
The MDA5 ifih1 (Catalog #MBS565076) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MDA5 monoclonal antibody (1B6) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s MDA5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~50kDa by Western blot. Researchers should empirically determine the suitability of the MDA5 ifih1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MDA5 ifih1 product has the following accession number(s) (GI #134047802) (NCBI Accession #Q9BYX4.3) (Uniprot Accession #Q9BYX4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MDA5 monoclonal antibody (1B6) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MDA5: Lane 1: MW Marker; Lane 2: HeLa; Lane 3: A431; Lane 4: L929; Lane 5: 3T3)
MDA5 is a highly conserved helicase whose ATPase activity can be stimulated by RNA and less potently by DNA. It contains two N-terminal CARD domains and a C-terminal helicase domain. The helicase domain, once freed from the CARD domains, then translocates from the cytoplasm to the nucleus where it acts on chromatin architecture. This could allow easier access of CAD, thereby accelerating the degradation of genomic DNA.
Immunogen: Recombinant human MDA5 (melanoma differentiation-associated gene 5) (aa 78-555). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MDA5 are readily searchable from our website. Different antibodies against the same target such as MDA5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Aicardi-Goutieres syndrome, Fever, Graves Disease, Inflammation, Influenza, Human, Liver Diseases, Lupus Erythematosus, Systemic, Necrosis, Nervous System Diseases, Psoriasis are some of the diseases may be linked to MDA5 monoclonal antibody (1B6). Blood, Bone, Heart, Kidney, Lung, Muscle, Pancreas, Skin, Thyroid, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. MDA5 also interacts with the following gene(s): DAK, DDX58, DDX60, IFIT1, MAVS, NLRX1, TLR3, UBC.