anti-Her2/Neu antibody product blog
Tags: Antibody; Monoclonal Antibody; HER2/neu; anti-HER2/neu antibody;
The Her2/Neu n/a (Catalog #MBS346013) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Her2/Neu Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Her2/Neu can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin.Dilution Range: 1:100 - 1:200. Researchers should empirically determine the suitability of the Her2/Neu n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Her2/Neu Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (IHC (paraffin) of human breast using Her2/Neu Antibody)
Her2 is a transmembrane tyrosine kinase receptor and part of the human epidermal growth factor receptor family that is expressed in a number of carcinomas. Overexpression of Her2 is often associated a number of aggressive types of breast cancer such as ductal breast cancer, as well as pulmonary and gastric adenocarcinomas.
Buffer: Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3. IHC Positive Control: Breast Carcinoma
IHC Suggested Protocol: 1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block � Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab � Dilute antibodies 1:100 � 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Her2/Neu are readily searchable from our website. Different antibodies against the same target such as Her2/Neu may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.