|The HIF-1 beta/ARNT arnt (Catalog #MBS2548961) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIF-1 beta/ARNT Monoclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s HIF-1 beta/ARNT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF).
IHC: 1:100-200. Researchers should empirically determine the suitability of the HIF-1 beta/ARNT arnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HIF-1 beta/ARNT arnt product has the following accession number(s) (GI #309747071) (NCBI Accession #NP_001184254.1) (Uniprot Accession #P27540). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1alpha and HIF-1beta, which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1alpha degradation and lead to its stabilization. In addition, HIF-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).HIF-1beta is also known as AhR nuclear translocator (ARNT) due to its ability to partner with the aryl hydrocarbon receptor (AhR) to form a heterodimeric transcription factor complex (8). Together with AhR, HIF-1beta plays an important role in xenobiotics metabolism (8). In addition, a chromosomal translocation leading to a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia (9). Studies also found that ARNT/HIF-1beta expression levels decrease significantly in pancreatic islets from patients with type 2 diabetes, suggesting that HIF-1beta plays an important role in pancreatic beta-cell function (10).
Immunogen: Recombinant Protein
Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol pH 7.4. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HIF-1 beta/ARNT are readily searchable from our website. Different antibodies against the same target such as HIF-1 beta/ARNT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.