anti-HIF1A antibody product blog
Tags: Antibody; Monoclonal Antibody; HIF1A; anti-HIF1A antibody; Hypoxia Inducible Factor 1a;
The HIF1A hif1a (Catalog #MBS602520) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hypoxia Inducible Factor 1a (HIF-1a) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Hypoxia Inducible Factor 1a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Dilution: Immunohistochemistry: 1:100-1:5000 (paraffin-embedded or frozen sections). Researchers should empirically determine the suitability of the HIF1A hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HIF1A hif1a product has the following accession number(s) (GI #15214788) (Uniprot Accession #Q16665). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hypoxia Inducible Factor 1a (HIF-1a) with the following immunoassay(s):
Western Blot (WB) (Western Blot of HIF1a in Cos7 cells treated or untreated with CoCl2 using MBS602520.)
Testing Data (Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using MBS602520.)
Immunohistochemistry (IHC) (Immunohistochemistry-Paraffin analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using MBS602520.)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using MBS602520.)
Immunofluorescence (IF) (Immunocytochemistry/Immunofluorescence detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using MBS602520.)
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein that activates gene transcription in response to reduced cellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, heme oxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is a heterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced by hypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulate hypoxia-inducible factors may be related to tumor-related angiogenesis in certain cancers.
Immunogen: Human HIF-1 alpha amino acids 329-530.
Cellular Localization: Nuclear. Positive Control: Multiforme glioblastoma. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HIF1A are readily searchable from our website. Different antibodies against the same target such as HIF1A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.