anti-Hif1a antibody product blog
Tags: Antibody; Monoclonal Antibody; HIF1A; anti-HIF1A antibody; HIF1 alpha;
The Hif1a hif1a (Catalog #MBS801921) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIF1 alpha Antibody: PerCP reacts with Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s HIF1 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB) (weak), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), IHC-Fr, IHC-P.1:1000-1:8000 (IHC), 8-21ug/mL (ICC/IF). Researchers should empirically determine the suitability of the Hif1a hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Hif1a hif1a product has the following accession number(s) (GI #226061948) (NCBI Accession #NP_034561.2) (Uniprot Accession #Q61221). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HIF1 alpha Antibody: PerCP with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis.)
Western Blot (WB) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Specific for HIF1Alpha
Scientific Background: Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1alpha is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1alpha is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1alpha degradation and lead to its stabilization. In addition, HIF-1alpha can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).