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anti-HIF1A antibody product blog

Posted on 2015-09-18 09:01:06 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; HIF1A; anti-HIF1A antibody;
The HIF1A hif1a (Catalog #MBS9200427) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIF1A Antibody (ascites) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s HIF1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB~~1:100~1000. Researchers should empirically determine the suitability of the HIF1A hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HIF1A hif1a product has the following accession number(s) (GI #340545531) (NCBI Accession #NP_001230013.1) (Uniprot Accession #Q16665). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HIF1A Antibody (ascites) with the following immunoassay(s):
Western Blot (WB) (HIF1A western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the HIF1A antibody detected the HIF1A protein (arrow).)
Western Blot (WB) HIF1A.

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Antigen Type: Recombinant Protein. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HIF1A are readily searchable from our website. Different antibodies against the same target such as HIF1A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. HIF1A also interacts with the following gene(s): ARNT, CREBBP, EGLN1, EGLN3, EP300, HIF1AN, HSP90AA1, TCEB1, UBC, VHL. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Prostate, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Brain Diseases, Breast Neoplasms, Carcinoma, Inflammation, Kidney Diseases, Liver Diseases, Lung Diseases, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to HIF1A Antibody (ascites).
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