anti-HIF1AN antibody product blog
Tags: Antibody; Monoclonal Antibody; HIF1AN; anti-HIF1AN antibody;
The HIF1AN hif1an (Catalog #MBS9230980) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIF1AN Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s HIF1AN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).WB: 1:4000. Researchers should empirically determine the suitability of the HIF1AN hif1an for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HIF1AN hif1an product has the following accession number(s) (GI #148596936) (NCBI Accession #NP_060372.2) (Uniprot Accession #Q9NWT6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HIF1AN Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-HIF1AN Antibody (C-term) at 1:4000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: ZR-75-1 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Hydroxylates HIF-1 alpha at \'Asp-803\' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Antigen Type: Recombinant Protein
Antigen Source: Human. Cellular Location: Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HIF1AN are readily searchable from our website. Different antibodies against the same target such as HIF1AN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.