anti-HIST1H3A antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-HIST1H3A antibody; HIST1H3A; Mono-methyl-Histone H3.1;
The HIST1H3A hist1h3a (Catalog #MBS7110774) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Mono-methyl-Histone H3.1 (K18) Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Mono-methyl-Histone H3.1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).WB: 1:500-1:2000
ICC: 1:50-1:500
IF: 1:30-1:200. Researchers should empirically determine the suitability of the HIST1H3A hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HIST1H3A hist1h3a product has the following accession number(s) (GI #4504281) (NCBI Accession #NP_003520.1) (Uniprot Accession #P68431). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given recombinant antibody. We\'ve tested the Mono-methyl-Histone H3.1 (K18) Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: Mono-methyl-Histone H3.1 (K18)antibody at 0.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa
)
Immunocytochemistry (ICC) (Immunocytochemistry analysis of MBS7110774 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (Immunofluorescence staining of Hela cells with MBS7110774 at 1:43, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunogen: A synthesized peptide. Conjugation: Non-conjugated
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The following patways have been known to be associated with this gene.