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anti-HIST1H3A antibody product blog

Posted on 2016-01-19 07:06:57 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-HIST1H3A antibody; HIST1H3A; Histone H3;
The HIST1H3A hist1h3a (Catalog #MBS9400124) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H3(mono-methyl-K79) Mouse Monoclonal Antibody (5G5) reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Western blotting: 1:500~1:2000. Researchers should empirically determine the suitability of the HIST1H3A hist1h3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HIST1H3A hist1h3a product has the following accession number(s) (GI #4504281) (NCBI Accession #NP_003520.1) (Uniprot Accession #P68431). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Histone H3(mono-methyl-K79) Mouse Monoclonal Antibody (5G5) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Hela cells, using diluted at 1) 1:1,000, 2) 1:3,000.)
Western Blot (WB) HIST1H3A.

Western Blot (WB) (Western blot analysis of 1) Rat Testis tissue, 2) Raw264.7, using diluted at 1:2000.)
Western Blot (WB) HIST1H3A.

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Immunogen Type: peptide
Immunogen Description: A synthetic Mono-methylated peptide corresponding to residues surrounding K79 of human histone H3. Modification: mono-methyl-K79
Target Name: Histone H3(mono-methyl-K79). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HIST1H3A are readily searchable from our website. Different antibodies against the same target such as HIST1H3A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Brain tissues are correlated with this protein.
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