anti-HISTIDINE TAG antibody product blog
Tags: Antibody; Monoclonal Antibody; HISTIDINE TAG; anti-HISTIDINE TAG antibody;
The HISTIDINE TAG n/a (Catalog #MBS211163) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s HISTIDINE TAG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).ELISA: Minimum Dilution: 1/20; Maximum Dilution: 1/1000;
Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/5000. Researchers should empirically determine the suitability of the HISTIDINE TAG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HISTIDINE TAG:HRP with the following immunoassay(s):
Testing Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin (MBS210332) and visualised with Streptavidin : HRP (MBS238416))
Testing Data (Western blot of HIS tagged protein probed with Mouse anti Histidine tag:Dylight800 (MBS212126D800GA). Visualised using a LI-COR Odyssey infrared imaging system)
Testing Data (Human anti Human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualised with Mouse anti-HIS tag: FITC antibody (MBS210678))
Testing Data (Western blot of HIS-tagged protein probed with Mouse anti Histidine tag (MBS216898) visualised with Rabbit anti Mouse IgG : HRP (MBS235192))
Testing Data (His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag (MBS212126))
Mouse anti Histidine tag antibody, clone AD1.1.10, recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Perservative Stabilisers: 0.01% Thiomersal
HRP Stabiliser
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: PAX6 transcription factor linked to histidine tag
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Synthetic Peptide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HISTIDINE TAG are readily searchable from our website. Different antibodies against the same target such as HISTIDINE TAG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.