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anti-HLA Class 1 Antigen A25,A26,A34(X-Reactive) antibody product blog

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Posted on 2016-02-19 18:08:30 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; HLA Class 1 Antigen A25,A26,A34(X-Reactive); anti-HLA Class 1 Antigen A25,A26,A34(X-Reactive) antibody;
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The HLA Class 1 Antigen A25,A26,A34(X-Reactive) n/a (Catalog #MBS600841) is an Antibody produced from Ascites fluid and is intended for research purposes only. The product is available for immediate purchase. The HLA Class 1 Antigen A25,A26,A34(X-Reactive) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s HLA Class 1 Antigen A25,A26,A34(X-Reactive) can be used in a range of immunoassay formats including, but not limited to, Microcytotoxicity Test.
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Recommended Dilution
Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the HLA Class 1 Antigen A25,A26,A34(X-Reactive) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HLA Class 1 Antigen A25,A26,A34(X-Reactive) with the following immunoassay(s):
Microcytotoxicity Test
Microcytotoxicity Test HLA Class 1 Antigen A25,A26,A34(X-Reactive).

The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans. The superlocus contains a large number of genes related to immune system function in humans. This group of genes resides on chromosome 6, and encodes cell-surface antigen-presenting proteins and many other genes. The HLA genes are the human versions of the MHC genes that are found in most vertebrates (and thus are the most studied of the MHC genes). The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplantations. The major HLA antigens are essential elements for immune function. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell (including viral peptides if present). These peptides are produced from digested proteins that are broken down in the proteasomes. The peptides are generally small polymers, about 9 amino acids in length. Foreign antigens attract killer T-cells (also called CD8 positive- or cytotoxic T-cells) that destroy cells.

HLA class II antigens (DP,DM, DOA,DOB,DQ, & DR) present antigens from outside of the cell to T-lymphocytes. These particular antigens stimulate T-helper cells to multiply, and these T-helper cells then stimulate antibody-producing B-cells to produce Antibodies to that specific antigen. Self-antigens are suppressed by suppressor T-cells.

Immunogen: HLA Class 1 Antigen-A25, A26, A34.
Cytotoxicity: Expected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
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