anti-HMGA2 antibody product blog
Tags: Antibody; Monoclonal Antibody; HMGA2; anti-HMGA2 antibody; High mobility group protein HMGI-C;
The HMGA2 hmga2 (Catalog #MBS225487) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The High Mobility Group Protein HMGI-C Antibody with Control Lysate reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s High Mobility Group Protein HMGI-C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:1000
Western Blotting: Detects a band of approximately 18 kDa in HepG2 cell lysates. Researchers should empirically determine the suitability of the HMGA2 hmga2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HMGA2 hmga2 product has the following accession number(s) (GI #1708263) (NCBI Accession #P52926.1) (Uniprot Accession #P52926). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the High Mobility Group Protein HMGI-C Antibody with Control Lysate with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of whole cell lysates probed with high mobility group protein HMGI-C antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemicDoc�� MP with 56 second exposure. Arrow points to high mobility group protein HMGI-C (molecular weight 18 kDa))
Immunogen: E Coli-derived recombinant protein corresponding to aa 1-109 of human high mobility group protein HMGI-C. Lysate Composition: 400ug HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution: If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HMGA2 are readily searchable from our website. Different antibodies against the same target such as HMGA2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Adenocarcinoma, Breast Neoplasms, Carcinoma, Cell Transformation, Neoplastic, Hematologic Diseases, LIPOMATOSIS, MULTIPLE, Lung Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Skin Diseases are some of the diseases may be linked to High Mobility Group Protein HMGI-C Antibody with Control Lysate. Bone, Connective Tissue, Embryonic Tissue, Heart, Liver, Lung, Mouth, Thyroid, Uterus, Vascular tissues are correlated with this protein.