anti-HO-1 antibody product blog
Tags: Antibody; Monoclonal Antibody; HO-1; anti-HO-1 antibody; Heme-Oxygenase-1;
The HO-1 hmox1 (Catalog #MBS190674) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Heme-Oxygenase-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoblot (IB).ELISA: Confirmed with antibody (1-10ug/ml) binding to HO-1 at 5ug/ml on the solid phase.
Immunoblotting: confirmed with antibody at 1.0-2.0ug/ml and 20ug of rat kidney lysate. A band of ~31kDa is detected. Researchers should empirically determine the suitability of the HO-1 hmox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HO-1 hmox1 product has the following accession number(s) (GI #4504437) (NCBI Accession #NP_002124.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Heme-Oxygenase-1 (HO-1) Monoclonal Antibody with the following immunoassay(s):
Testing Data
Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.
Antigen: His-tagged rat heme-oxygenase-1 (HO-1), Accession no. NP_036712.1. Positive Control: Rat kidney lysate.
Dilution Instructions: Dilute in PBS or medium that is identical to that used in the assay system. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HO-1 are readily searchable from our website. Different antibodies against the same target such as HO-1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. HO-1 also interacts with the following gene(s): AKT1, BLVRA, BLVRB, FOS, HIF1A, JUN, MAPK14, NFE2L2, USF2. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Skin, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Drug-Induced Liver Injury, Fibrosis, Hypertension, Inflammation, Liver Diseases, Lung Injury, Myocardial Ischemia, Neurodegenerative Diseases, Reperfusion Injury, Wounds and Injuries are some of the diseases may be linked to Heme-Oxygenase-1 (HO-1) Monoclonal Antibody.