anti-HPRT antibody product blog
Tags: Antibody; Monoclonal Antibody; Hypoxanthine-Guanine Phosphoribosyltransferase; HPRT; anti-HPRT antibody;
The HPRT hprt1 (Catalog #MBS140407) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Hypoxanthine-Guanine Phosphoribosyltransferase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).HPRT antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500~1:5000.Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the HPRT hprt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HPRT hprt1 product has the following accession number(s) (GI #4504483) (NCBI Accession #NP_000185.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Introduction: HPRT1 has a main part in the generation of purine nucleotides through the purine salvage pathway. HPRT1 primarily functions to salvage purines from degraded DNA to renewed purine synthesis. Therefore, it performs as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.
Immunogen: Anti-human HPRT mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HPRT amino acids 1-218 purified from E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HPRT are readily searchable from our website. Different antibodies against the same target such as HPRT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Cell Transformation, Neoplastic, Chromosome Aberrations, Hyperuricemia, Kidney Diseases, Lesch-Nyhan Syndrome, Liver Diseases, Liver Neoplasms, Lung Diseases, Neoplasms, Experimental are some of the diseases may be linked to Mouse Anti Human Hypoxanthine-Guanine Phosphoribosyltransferase. HPRT also interacts with the following gene(s): ADSS, APRT, ATIC, GDA, GMPS, PNP, TPMT, XDH. The following patways have been known to be associated with this gene.