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anti-HSP60 antibody product blog

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Posted on 2018-02-01 00:24:00 by mybiosource_staff
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Tags: Antibody; HSP60; Monoclonal Antibody; anti-Hsp60 antibody;
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The HSP60 hspd1 (Catalog #MBS2549941) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP60 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s HSP60 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin (IHC-p).
IHC: 1:200-1:300. Researchers should empirically determine the suitability of the HSP60 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HSP60 hspd1 product has the following accession number(s) (GI #31542947) (NCBI Accession #NP_002147.2) (Uniprot Accession #P10809). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HSP60 Monoclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer tissue labeling HSP60 Monoclonal Antibody with E-PA-3670 at dilution of 1:200. Heat induced epitope retrieval was performed by boiling the tissue sections in citrate buffer(PH6.0)for 20 minutes. Incubation with the primary antibody was performed for overnight at 4 degree C followed by keeping at room temperature for 15mins. HRP conjugated Anti-Mouse secondary antibody was applied for 30 minutes at room temperature followed by DAB as chromogen, thencounterstainwithhaematoxylin.)
Immunohistochemistry (IHC) HSP60.

Immunohistochemistry (IHC) (Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer tissue labeling HSP60 Monoclonal Antibody with E-PA-3670 at dilution of 1:200. Heat induced epitope retrieval was performed by boiling the tissue sections in citrate buffer(PH6.0)for 20 minutes. Incubation with the primary antibody was performed for overnight at 4 degree C followed by keeping at room temperature for 15mins. HRP conjugated Anti-Mouse secondary antibody was applied for 30 minutes at room temperature followed by DAB as chromogen, thencounterstainwithhaematoxylin.)
Immunohistochemistry (IHC) HSP60.

HSPs is a group of stress protein cells induced by stress stimulation, and it is related to tumorigenesis, proliferation and differentiation. HSPs can be classified into 3 types according to their different molecular weight, and the distribution and function of each type of HSPs are different. HSP60 is mainly used in research of tongue squamous cell carcinoma, colon cancer, pancreatic cancer and breast cancer, which may have prognostic significance.

Function: Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Immunogen: Full length protein from eukaryotic expression system.
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Conjugation: Unconjugated.
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