anti-Hsp70 antibody product blog
Tags: Antibody; HSP70; Monoclonal Antibody; anti-HSP70 antibody;
The Hsp70 hsp70 (Catalog #MBS190640) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Hsp70 can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB), ELISA (EIA).Immunoblotting: Use at 1ug/ml. A band of ~70kDa is detected.
ELISA: Use at 1-10ug/ml with Hsp70 sample on the solid phase. Researchers should empirically determine the suitability of the Hsp70 hsp70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Hsp70 hsp70 product has the following accession number(s) (GI #829026803) (NCBI Accession #AKL59498.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Hsp70 genes encode heat-inducible 70kDa heat shock proteins. Hsp70 binds ATP with high affinity and possesses weak ATPase activity which can bze stimulated by binding to unfolded proteins and synthetic peptides. The structure of the ATP binding domain displays multiple features of nucleotide binding proteins. The binding of ATP triggers a critical conformational change which leads to the release of the bound substrate protein. The universal ability of Hsp70s to undergo cycles of binding to and release from partially unfolded proteins is the basis of their role in protein synthesis, protein folding, oligomerization, and protein transport.
Antigen: Purified bovine Hsp70 (accession no. NP_776975.1). Positive Control: HeLa cell lysate.
Dilution Instructions: Dilute in PBS or medium which is identical to that used in the assay system. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Hsp70 are readily searchable from our website. Different antibodies against the same target such as Hsp70 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.