|The HSP70 hspa1a (Catalog #MBS808354) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP70 Antibody, Clone 1H11: PE/ATTO 594 reacts with Human; Mouse; Rat; Bovine; Pig and may cross-react with other species as described in the data sheet. MyBioSource\'s HSP70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS).
FC/FACS: 1:250. Researchers should empirically determine the suitability of the HSP70 hspa1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSP70 hspa1a product has the following accession number(s) (GI #194248072) (NCBI Accession #NP_005336.3) (Uniprot Accession #P0DMV8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HSP70 Antibody, Clone 1H11: PE/ATTO 594 with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.)
Immunofluorescence (IF) (Fluorescence-activated cell sorting analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Tissue: Jurkat E6.1 cells. Species: Human. Fixation: No fixation. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 20 ug/ml for 40 min at 4 degree C. Counterstain: Propidium Iodide nuclear stain at 2.5 ug/ml for 5 min at RT. Isotype Control: Anti-mouse FITC at 1:32 for 15 min at RT (blue line). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.)
HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6).
Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9).
Anti-HSP70 antibody, clone 1H11 is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.