anti-HSP86; Heat shock protein 90 beta antibody product blog
Tags: Antibody; Monoclonal Antibody; Hsp90 beta; anti-HSP86; Heat shock protein 90 beta antibody; HSP86; Heat shock protein 90 beta ;
The HSP86; Heat shock protein 90 beta hsp90ab1 (Catalog #MBS565009) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP90 beta monoclonal antibody (K3705) reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Porcine, Sheep and may cross-react with other species as described in the data sheet. MyBioSource\'s HSP90 beta can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB).Immunohistochemistry (1:50)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~90kDa by Western blot. Researchers should empirically determine the suitability of the HSP86; Heat shock protein 90 beta hsp90ab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSP86; Heat shock protein 90 beta hsp90ab1 product has the following accession number(s) (GI #17865718) (NCBI Accession #P08238.4) (Uniprot Accession #P08238). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HSP90 beta monoclonal antibody (K3705) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of HSP90 beta : Lane 1: MW marker, Lane 2: HSP90 Native Human Protein, Lane 3: HSP90 alpha Recombinant Human Protein, Lane 4: HSP90 beta Recombinant Human Protein, Lane 5: HeLa, Lane 6: PC-12, Lane 7: CHO-K1, Lane 8: 3T3.)
The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.
Immunogen: Recombinant human HSP90 beta. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSP86; Heat shock protein 90 beta are readily searchable from our website. Different antibodies against the same target such as HSP86; Heat shock protein 90 beta may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.