anti-HSP90AB1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-HSP90AB1 antibody; HSP90AB1; HSP90 ;
The HSP90AB1 hsp90ab1 (Catalog #MBS800920) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp90 complex Antibody: ATTO 488 reacts with Human, Mouse, Rat, Rabbit and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp90 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP).Best results for IP associated with use of goat anti-mouse IgM beads. Researchers should empirically determine the suitability of the HSP90AB1 hsp90ab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSP90AB1 hsp90ab1 product has the following accession number(s) (GI #20149594) (NCBI Accession #NP_031381.2) (Uniprot Accession #P08238). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsp90 complex Antibody: ATTO 488 with the following immunoassay(s):
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3. Tissue: reticulocyte lysate. Species: Rabbit. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody at 1:1000. Hsp90 complex isolated by immunoprecipitation.)
Background Info: Immunoprecipitates 90kDa proteins corresponding to the molecular mass of Hsp90. Co-immunoprecipitates Hsp90 complexes, including Hsp70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2alpha (eIF-2alpha) kinase (HRI).
Scientific Background: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1-2% of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (7).