anti-HSPA1A antibody product blog
Tags: Antibody; HSP70; Monoclonal Antibody; HSPA1A; anti-HSPA1A antibody;
The HSPA1A hspa1a (Catalog #MBS803104) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GIT1 Antibody: ATTO 390 reacts with Human, Bovine, Mouse, Rat, Porcine and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).1ug/ml WB. Researchers should empirically determine the suitability of the HSPA1A hspa1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The HSPA1A hspa1a product has the following accession number(s) (GI #40254806) (NCBI Accession #NP_776975.1) (Uniprot Accession #Q27975). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the GIT1 Antibody: ATTO 390 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:1000.)
Background Info: Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70 on SDS Page immunoblots. Does not cross-react with Hsc70.
Scientific Background: G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
Conjugate: ATTO 390. Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HSPA1A are readily searchable from our website. Different antibodies against the same target such as HSPA1A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.