Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-HSPA2 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-CD4 antibody blog
 • Il1rl1 blocking peptide blog
 • anti-RANBP9 antibody blog
 • anti-ICAM-1 antibody blog
 • anti-KRT8 antibody blog
 • anti-PPP2CA antibody blog
 • C4a blocking peptide blog
 • anti-SGCD antibody blog
 • anti-CD276 antibody blog
 • anti-ARID3A antibody blog
 • S100A9 antibody blog
 • anti-PIM1 antibody blog
 • anti-CD25 antibody blog
 • anti-ECFP antibody blog
 • anti-APBB1IP antibody blog
 • anti-CD18a antibody blog
 • anti-GJB2 antibody blog
 • anti-PXN antibody blog
 • anti-BTCry mAb1 antibody blog
 • anti-TCR gamma delta antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Adiponectin (ADIPOQ) ELISA Kit
 • Glutamate receptor delta-2 subunit (GRID2) ELISA Kit
 • Thyroid hormone receptor beta (THRB) ELISA Kit
 • TRABID-ZRANB1 Antibody
 • TARS Antibody
 • TNF receptor-associated factor 6 (TRAF6) ELISA Kit
 • PDLIM5 Antibody
 • adaptor-related protein complex 3, delta 1 subunit Antibody
 • Neurofilament M,H (NEFL) Antibody
 • Alectinib Inhibitor
 • Glycogen Phosphorylase, Liver Form (PYGL) Antibody
 • Macrophage Inflammatory Protein-1a (MIP-1 a) Antibody
 • ABL1-Y204 Antibody
 • Anti-myocardial antibody (IgM) (AMA (IgG) ) ELISA Kit
 downarrow more ...
separator

anti-HSPA2 antibody product blog

separator
Posted on 2015-12-30 09:55:31 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Monoclonal Antibody; Hsp70/Hsc70; HSPA2; anti-HSPA2 antibody;
separator
The HSPA2 hsp70 (Catalog #MBS800955) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp70/Hsc70 Antibody: ATTO 700 reacts with Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast and may cross-react with other species as described in the data sheet. MyBioSource\'s Hsp70/Hsc70 can be used in a range of immunoassay formats including, but not limited to, Westen Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Protein-binding Assay.
1ug/ml was sufficient for detection of hsp70/Hsc70 in 20ug of HeLa lysate. Researchers should empirically determine the suitability of the HSPA2 hsp70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The HSPA2 hsp70 product has the following accession number(s) (GI #55742654) (NCBI Accession #NP_001006686.1) (Uniprot Accession #P08106). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsp70/Hsc70 Antibody: ATTO 700 with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)
Immunohistochemistry (IHC) HSPA2.

Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.)
Immunohistochemistry (IHC) HSPA2.

Western Blot (WB) (Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.)
Western Blot (WB) HSPA2.

Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)
Immunocytochemistry/Immunofluorescence (ICC/IF) HSPA2.

Western Blot (WB) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.)
Western Blot (WB) HSPA2.

Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)
Immunohistochemistry (IHC) HSPA2.

Background Info: Detects ~72 and ~73kDa proteins corresponding to the molecular mass of inducible Hsp and Hsc on SDS PAGE immunoblots.

Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions